ß-Glucuronidases & Arylsulfatases

B-One™, BGTurbo Glycerol Free & BGS™

Recombinant ß-Glucuronidases and arylsulfatases for improved analytical efficiency and higher sample throughput

The detection of drug and medication abuse is never just routine. Each analyte requires the best possible sample preparation. With the ß-glucuronidase and arylsulfatase solutions from finden KURA, we provide the appropriate enzymes for your lab. The recombinant products have been developed to rapidly hydrolyze conjugated metabolites in urine samples. The treated samples are ready for use in just a few steps

Product characteristics

The recombinant design of the KURA products ensures consistent lot-to-lot quality
The enzymes‘ high purity protects your columns, thus extending their lifetime
No interference through secondary enzymes and contaminants
Short incubation times at room temperature facilitate automated workflow
Particularly suitable for laboratories with a high sample throughput
Long shelf life – 12 months at 2-8°C or up to 3 months at ambient temperature

Background

Both glucuronidation and sulfation play an important role in the human body. They are particularly important in the metabolism of various medications, drugs, and hormones, e.g., steroids. Both increase the solubility of substances, thus facilitating renal excretion.

However, glucuronides and sulfates impair direct analysis and quantification. To be able to detect the free analytes, ß-glucuronidases and arylsulfatases are used to cleave the conjugates from the metabolite, converting them back to the original substance.

Clinical and forensic laboratories use these enzymes to ensure accurate results when quantifying analytes by mass spectrometry, for example in urine. During this process, the efficiency of both ß-glucuronidase and arylsulfatase is highly dependent on the source and production process of the respective enzymes.

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